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To further elucidate the chemical mechanism of binding, EPI-054 (chlorohydrin) or inactive EPI-063 (no chlorohydrin) was incubated with purified recombinant AF1 protein under cell-free conditions prior to Click-chemistry to add fluorescein…

Here we identify cyclin-dependent kinase 1 (Cdk1, also called Cdc2) as an AR Ser-81 kinase. Cdk1 transfection increased Ser-81 phosphorylation and AR expression, whereas Cdk1 inhibitors markedly decreased AR Ser-81 phosphorylation, protein… Sequencing confirmed that it encoded the mouse homologue of human EST product. Using this cDNA fragment as a template, a DIG-labeled probe was prepared using a DIG High Prime Labeling Kit ( Boehringer Mannheim GmbH, Mannheim, Germany), and… Mesenchymal–epithelial interactions played an important role in development, as has been described in experimental animals. Cells were transfected by calcium phosphate precipitation, as described (6). All transfections included 0.2 μg of p6RlacZ (19) for normalization of transfection efficiency. Address all correspondence to Gordon C. Shore, Department of Biochemistry, McIntyre Medical Sciences Building, McGill University, Montreal, QC, Canada H3G 1Y6. Tel.: (514) 398-7282; Fax: (514) 398-7384; E-mail: shore{at}medcor.mcgill.ca

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